. Kuyinto ubuchwepheshe obuphakeme obuphezulu obuthuthukisiwe ngokususelwa ekuhlaziyeni nasekuhlelweni kwamangqamuzana athile. Ingakwazi ukukala ngokushesha futhi ihlukanise usayizi, ukwakheka kwangaphakathi, i-DNA, i-RNA, amaprotheni, ama-antigen nezinye izintengile ezingokomzimba noma zamakhemikhali amaseli, futhi kungasuselwa ekuqoqweni kwalezi zigaba.
I-Flow Cytometer ikakhulukazi iqukethe izingxenye ezinhlanu ezilandelayo:
1 igumbi lokugeleza kanye nohlelo lwe-fluidics
2 I-Laser Light Source and Beam System System
3 Optical System
4 Electronics, Isitoreji, Isibonisi Nokuhlaziya Uhlelo
Uhlelo lokuhlunga lweseli engu-5
Phakathi kwazo, ukuthokozisa kwe-laser emthonjeni wokukhanya kwe-laser kanye no-beam ukwakha uhlelo kungukulinganisa okuyinhloko kwezimpawu ze-fluorescence ku-flow cytometry. Ukuqina kokukhanya okujabulisayo nesikhathi sokuvezwa kuhlobene nokuqina kwesiginali ye-fluorescence. I-laser ingumthombo ohambisanayo wokukhanya ongahlinzeka nge-wavelength eyodwa, ubukhulu obukhulu, kanye nokukhanya okuphezulu. Kungumthombo ofanele wokukhanya ongcono wokuhlangabezana nalezi zidingo.
Kunamalensi amabili ama-cylindrical phakathi komthombo we-laser negumbi lokugeleza. Lawa ma-lens agxile emgodini we-laser onesigaba sesiphambano esiyindilinga esikhishwe emthonjeni we-laser emgodini we-elliptical ngesigaba esincane (22 μm μm). Amandla we-laser ngaphakathi kwalolu bam we-elliptical asatshalaliswa ngokuya ngokusatshalaliswa okujwayelekile, ukuqinisekisa ukunxusa okuguquguqukayo okungaguquki kwamaseli adlula endaweni yokutholwa kwe-laser. Ngakolunye uhlangothi, uhlelo lwe-optical luqukethe amasethi amaningi amalensi, ama-mainhones, kanye nezihlungi, ezingahlukaniswa cishe zaba ngamaqembu amabili: Upstream futhi wehle kakhulu egumbini lokugeleza.
Uhlelo lokubona phambi kwegumbi lokugeleza liqukethe i-lens ne-pinhole. Umsebenzi oyinhloko we-lens ne-pinhole (imvamisa amalensi amabili kanye ne-pinhole) ukugxila ugongolo lwe-laser ngesigaba sesilinganiso esiyindilinga esikhishwe yi-laser emgqeni we-eltiptical esigabeni esincane. Lokhu kusabalalisa amandla we-laser ngokusho kokusatshalaliswa okujwayelekile, ukuqinisekisa ukuqina kokukhanya okungaguquki kwamaseli endaweni yokutholwa kwe-laser kanye nokunciphisa ukuphazanyiswa ukukhanya okukhanyayo.
Kunezinhlobo ezintathu eziphambili zokuhlunga:
1: I-Long Pass Filter (LPF) - Ivumela kuphela ukukhanya ngama-wavelength aphezulu kunenani elithile elizodlula kulo.
2: I-Shot-Pass Filter (SPF) - Ivumela kuphela ukukhanya ngama-wavelength angezansi kwenani elithile ukuze udlule.
I-3: I-bandpass fingerving (BPF) - Ivumela kuphela ukukhanya kububanzi obuthile be-wavelength ukuze udlule.
Ukuhlanganiswa okuhlukile kokuhlunga kungaqondisa amasignali we-fluorescence kuma-wavelength ahlukene kumashubhu we-photomultipl ngamunye (PMTS). Isibonelo, izihlungi zokuthola ama-fluorescence eziluhlaza (i-FITC) phambi kwe-PMT yi-LPF550 ne-BPF525. Ukuhlunga okusetshenziselwa ukuthola i-orange-red fluorescence (pe) phambi kwe-PMT yi-LPF600 ne-BPF575. Izihlungi zokuthola i-Red Fleorescence (CY5) phambi kwe-PMT yi-LPF650 ne-BPF675.
I-Flow Cytometry isetshenziselwa ukuhlela kweseli. Ngokuthuthuka kobuchwepheshe bekhompyutha, ukuthuthukiswa kwe-immunology kanye nokusungulwa kwe-monoclonal antibody technology, izinhlelo zokusebenza zayo ku-biology, umuthi, ikhemisi nezinye izinkambu ziya ngokuya zisaba kakhulu. Lezi zinhlelo zokusebenza zifaka ukuhlaziywa kwamaselula, i-apoptosis yeselula, ukuthayipha kwamaseli, ukuxilongwa kwesigigaba, ukuhlaziywa kwezidakamizwa, njll.
Isikhathi Sokuposa: Sep-21-2023
